This investigation tests how well the enzyme amylase performs at different pH values. Amylase is a carbohydrase enzyme made by the pancreas and by glands in and around the mouth and throat. It helps ...

pH is a critical parameter in method development and routine enzyme assay measurement. At the ideal pH value, called optimal pH, enzymes have their highest activity. Accurate and reliable ...

This article describes a simple method to determine the optimum pH for the activity of one enzyme in a coupled enzyme assay. Decoupling of the two-step reaction is achieved by heat denaturing the ...