Researchers describe a new measurement and imaging approach that can resolve nanostructures smaller than the diffraction limit of light without requiring any dyes or labels. The work is a modification ...

Stimulated emission depletion (STED) microscopy has shown that diffraction-unlimited spatial resolution is viable with conventional lenses and visible light. Stefan W Hell, Lars Kastrup and Katrin I ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

Raman microscopy combines optical microscopy with the ability to determine the chemical makeup of surfaces. Laser light is delivered to the sample surface via the same objective as the optical light ...

Fluorescence light microscopy techniques offer several advantages when imaging biological samples, including high image contrast, good labeling specificity, multicolor, and three dimensional (3D) ...

A research team has created a fluorescent marker molecule that does not degrade under a STED microscope: the photobleaching problem has been solved. Images of living cells can now be captured over ...

Nanoscopy is a field of microscopy that focuses on imaging and studying structures and processes at the nanoscale, typically below the diffraction limit of light. It encompasses various techniques ...

Three-dimensional imaging is dramatically expanding the ability of researchers to examine biological specimens, enabling a peek into their internal structures. And recent advances in X-ray diffraction ...

You can tell a lot about a material based on the type of light shining at it: Optical light illuminates a material's surface, ...

Conceptualized in 1990, it was six years later at the University of Turku that Stephen Hell and his colleagues developed STED microscopy. As STED is able to produce imaging beyond the diffraction ...