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引言 我们长期信奉着一条近乎真理的中心法则：DNA转录为mRNA，mRNA翻译为蛋白质。在这种线性的逻辑下，mRNA往往被视为蛋白质的忠实代理人。过去十年，单细胞测序技术（scRNA-seq）的爆发，让我们习惯于通过统计mRNA的数量来推断细胞的功能状态，仿佛掌握了剧本（转录本），就等于看到了演出（蛋白质功能）。 然而，剧本真的等于演出吗？